精品无码久久久久久久动漫,国产亚洲精品久久久999蜜臀,免费无码成人AV电影在线播放,白嫩少妇激情无码

您好,歡迎進(jìn)入研域(上海)化學(xué)試劑有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
  • 資料下載DOWN

    您當(dāng)前的位置:首頁(yè) > 資料下載 > 人活化凝血因子Ⅻ(FⅫa)英文說(shuō)明書(shū)

    人活化凝血因子Ⅻ(FⅫa)英文說(shuō)明書(shū)

    發(fā)布時(shí)間: 2017/7/26  點(diǎn)擊次數(shù): 828次
    提 供 商: 研域(上海)化學(xué)試劑有限公司 資料大?。?/td>
    圖片類型: 下載次數(shù): 98
    資料類型: DOC 瀏覽次數(shù): 828
    相關(guān)產(chǎn)品:
    詳細(xì)介紹: 文件下載    

                   Human Activated coagulation factor Ⅻ (FⅫa)FOR
    RESEARCH USE ONLY

    Assay range:2.2U/L - 80U/L               96 determinations
    Purpose
    This kit allows for the determination of FⅫa concentrations in Human serum, cell culture supernates and other biological fluids

    Principle of the assay
    The kit assay Human FⅫa level in the sample, use Purified Human FⅫa antibody to coat microtiter plate wells, make solid-phase antibody, then add FⅫa to wells, Combined FⅫa antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human FⅫa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Materials provided with the kit
    1    wash  solution    20ml×1bottle    7    Stop Solution    6ml×1 bottle
    2    HRP-Conjugate reagent    6ml×1 bottle    8    Standard(160U/L)    0.5ml×1 bottle
    3    Microelisa stripplate    12well×8strips    9    Standard diluent    1.5ml×1bottle
    4    Sample diluent    6ml×1 bottle    10    Instruction    1
    5    Chromogen Solution A    6ml×1 bottle    11    Closure plate membrane    2
    6    Chromogen Solution B    6ml×1 bottle    12    Sealed bags    1
    Specimen requirements
    1.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
    2.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
    Assay procedure
    1.Dilute and add sample:Dilute Original density Standard as follow table:
    80U/L    5 Standard    150μl Original density Standard+150μl Standard diluent
    40U/L    4 Standard    150μl 5 Standard+150μl Standard diluent
    20U/L    3 Standard    150μl 4 Standard+150μl Standard diluent
    10U/L    2 Standard    150μl 3 Standard +150μl Standard diluent
    5U/L    1 Standard    150μl 2 Standard +150μl Standard diluent
    2. Add sample: Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
    3. Incubate:  After closing plate with Closure plate membrane , incubate for 30 min at 37℃.
    4. Configurate liquid: 30-fold(or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
    5. Washing: Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
    6. Add enzyme: Add HRP-Conjugate reagent 50μl to each well, except  blank well. 
    7. Incubate: Operation with 3.
    8. Washing: Operation with 5.
    9. Color: Add Chromogen Solution A 50ul and Chromogen Solution B 50ul to each well, evade the light preservation for 10 min at 37℃
    10. Stop the reaction: Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
    11. Assay: take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
    Steps description
    Standard, Sample diluent


    Add Standard, Sample diluent, incubate for 30 min at 37℃.


    Wash 5 time,Add HRP-Conjugate reagent, incubate for 30 min at 37℃.


    Wash 5 times,Add Chromogen Solution A and B, incubate for 10 min at 37℃.


    Add Stop Solution


    Read absorbance at 450nm within 15 min


    calculate
    Calculate
    Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
    Important notes
    1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
    2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
    3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
    4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
    5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
    6.The substrate evade the light preservation.
    7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
    8.All samples, washing buffer and each kind of reject should according to infective material process.
    9.Do not mix reagents with those from other lots.

    Storage and validity
    1.Storage:  2-8℃.
    2.validity: six months

產(chǎn)品中心 Products
在線客服 聯(lián)系方式

服務(wù)熱線

021-54479081
021-54461587

无码天堂亚洲国产av久久| 日本三线免费视频观看| 人妻换人妻仑乱| 一区二区三区内射美女毛片| 国产午夜福利100集发布| gv天堂gv无码男同在线观看| 亚洲精品美女久久7777777| 亚洲 欧美 中文 日韩aⅴ| WWW性久久久COM| 色狠狠av一区二区三区| 国产sm鞭打折磨调教视频| 日韩一线无码AV毛片免费| 国产精品久久久久久亚洲| 麻豆国产人妻欲求不满谁演的| 欧美熟妇大荫蒂高潮视频| 他揉捏她两乳不停呻吟人妻| 日韩欧美亚洲国产精品字幕久久久 | 精品久久久久久无码人妻蜜桃| 国产18禁黄网站免费观看| 亚洲精品99久久久久中文字幕| 午夜不卡av免费| 国产 亚洲熟妇| 精品乱码一区二区三区四区| 欧美激情无码视频一二三| 国产精品综合一区二区三区| 久久久久亚洲av无码专区体验 | 国产精品亚洲欧美大片在线观看| 久久久久免费毛a片免费| 国产偷人爽久久久久久老妇app| 国产日韩精品中文字无码| 久久久久久AV无码| 三上悠亚被弄到痉挛惨叫视频| 色噜噜综合亚洲AV中文无码 | 亚洲AV成人无遮挡网站在线观看| 香蕉久久精品日日躁夜夜躁| 乱码午夜-极国产极内射| 亚洲最大成人网站| 初六苏梅全文免费阅读| 2012中文字幕| 久久久久99精品国产片| 欧美最猛黑人xxxx黑人猛交|